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zetaprobe gt nylon membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad zetaprobe gt nylon membrane
    Zetaprobe Gt Nylon Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zetaprobe+gt+nylon+membranes/Zeta-Probe+GT+Membrane/pmc09266788-216-35-39
    Average 96 stars, based on 1714 article reviews
    zetaprobe gt nylon membrane - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Differential expression and interaction of transcription co-activator MBF1 with TATA-binding protein (TBP) in the apicomplexan Cryptosporidium parvum.
    Article Snippet: Received 6 November 2003 Revised 18 February 2004 Accepted 18 February 2004 All gene-specific transcriptional activators initiate gene transcriptions by binding to promoter sequences and recruiting general transcription factors including TATA-binding protein (TBP) to upstream of targeted genes.. Some of them require multiprotein bridging factors (MBFs); for example, the type 1 MBF (MBF1) which interconnects the gene activator with TBP.. In this study, the properties of a previously cloned type 1 multiprotein bridging factor (CpMBF1) and a newly identified TBP (CpTBP1) from the apicomplexan Cryptosporidium parvum were investigated.

    Article Title: Tyramide-FISH mapping of single genes for development of an integrated recombination and cytogenetic map of chromosome 5 of Allium cepa.
    Article Snippet: Chromosome 5 of onion carries major quantitative trait loci (QTL) of interest to breeders that control dry-matter content, pungency and storability of bulbs, amounts and types of epicuticular waxes, and resistances to abiotic factors.. SNPs, SSRs and RFLPs in expressed regions of the onion genome have been genetically mapped and we used these clones and sequences from the NCBI database to develop DNA probes for in situ hybridization to integrate the genetic and physical maps of onion chromosome 5.. We produced genomic amplicons from expressed regions of the onion genome that carried both exons and introns to increase the hybridization specificity of the probes and to enlarge the target DNA sizes.

    Fractionation:

    Article Title: A lupus-like syndrome develops in mice lacking the Ro 60-kDa protein, a major lupus autoantigen
    Article Snippet: For Northern blot analyses to detect Y RNAs, total brain tissue was lysed in TRIzol (Invitrogen), and RNA was isolated as described by the supplier. .. After fractionation in 5% polyacrylamide/8 M urea gels, the RNA was transferred to Zetaprobe GT nylon membranes (Bio-Rad) in 0.5× TBE (1× TBE = 89 mM Tris/89 mM boric acid/2.0 mM EDTA, pH 8.3) at 150 mA for 16 h. Oligonucleotides used to detect mY1 and mY3 RNA were 5′-AAGGGGGGAAAGTGTAGAACAGGA-3′ and 5′-GAGCGGAGA AGGA ACA A AGA A ATCTG-3′, respectively. ..

    Southern Blot:

    Article Title: Regulator of sex-limitation ( Rsl ) encodes a pair of KRAB zinc-finger genes that control sexually dimorphic liver gene expression
    Article Snippet: .. For Southern blotting, liver DNA was isolated and 10 μg was restriction-digested, electrophoresed through agarose, and transferred to ZetaProbe GT Nylon Membranes (Bio-Rad) by standard procedures ( Ausubel et al. 1993 ). .. PCR products for Rslcan-4 and Rslcan-9 from mouse strains B10.D2, B6, B10.D2.PL, 129, and B10.D2.FM were cloned into either pCR2.1-TOPO (Invitrogen) or pGEM-T-Easy (Promega), and were sequenced with T7 and Sp6 vector primers by the University of Michigan Sequencing Core Facility.

    Article Title: Regulator of sex-limitation ( Rsl ) encodes a pair of KRAB zinc-finger genes that control sexually dimorphic liver gene expression
    Article Snippet: .. For Southern blotting, liver DNA was isolated and 10 µg was restriction-digested, electrophoresed through agarose, and transferred to ZetaProbe GT Nylon Membranes (Bio-Rad) by standard procedures ( Ausubel et al. 1993 ). .. PCR products for Rslcan-4 and Rslcan-9 from mouse strains B10.D2, B6, B10.D2.PL, 129, and B10.D2.FM were cloned into either pCR2.1-TOPO (Invitrogen) or pGEM-T-Easy (Promega), and were sequenced with T7 and Sp6 vector primers by the University of Michigan Sequencing Core Facility.

    Isolation:

    Article Title: Regulator of sex-limitation ( Rsl ) encodes a pair of KRAB zinc-finger genes that control sexually dimorphic liver gene expression
    Article Snippet: .. For Southern blotting, liver DNA was isolated and 10 μg was restriction-digested, electrophoresed through agarose, and transferred to ZetaProbe GT Nylon Membranes (Bio-Rad) by standard procedures ( Ausubel et al. 1993 ). .. PCR products for Rslcan-4 and Rslcan-9 from mouse strains B10.D2, B6, B10.D2.PL, 129, and B10.D2.FM were cloned into either pCR2.1-TOPO (Invitrogen) or pGEM-T-Easy (Promega), and were sequenced with T7 and Sp6 vector primers by the University of Michigan Sequencing Core Facility.

    Article Title: Regulator of sex-limitation ( Rsl ) encodes a pair of KRAB zinc-finger genes that control sexually dimorphic liver gene expression
    Article Snippet: .. For Southern blotting, liver DNA was isolated and 10 µg was restriction-digested, electrophoresed through agarose, and transferred to ZetaProbe GT Nylon Membranes (Bio-Rad) by standard procedures ( Ausubel et al. 1993 ). .. PCR products for Rslcan-4 and Rslcan-9 from mouse strains B10.D2, B6, B10.D2.PL, 129, and B10.D2.FM were cloned into either pCR2.1-TOPO (Invitrogen) or pGEM-T-Easy (Promega), and were sequenced with T7 and Sp6 vector primers by the University of Michigan Sequencing Core Facility.

    Agarose Gel Electrophoresis:

    Article Title: Tyramide-FISH mapping of single genes for development of an integrated recombination and cytogenetic map of chromosome 5 of Allium cepa.
    Article Snippet: Chromosome 5 of onion carries major quantitative trait loci (QTL) of interest to breeders that control dry-matter content, pungency and storability of bulbs, amounts and types of epicuticular waxes, and resistances to abiotic factors.. SNPs, SSRs and RFLPs in expressed regions of the onion genome have been genetically mapped and we used these clones and sequences from the NCBI database to develop DNA probes for in situ hybridization to integrate the genetic and physical maps of onion chromosome 5.. We produced genomic amplicons from expressed regions of the onion genome that carried both exons and introns to increase the hybridization specificity of the probes and to enlarge the target DNA sizes.



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